Western blot analysis of PIK3C2A in HT29 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with PIK3C2A (Product # MA5-26506) monoclonal antibody at a dilution of 1:500.

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a PIK3C2A monoclonal antibody (Product # MA5-26506).